A sensitive method for quantitative microdetermination of lipids
نویسندگان
چکیده
منابع مشابه
A method for microdetermination of major neutral lipids and an application of the procedure to tissue lipids.
Cholesterol, cholesteryl esters, triglycerides and fatty acids as major neutral lipids and phospholipids were examined in quantitative analysis. The method consisted of three steps: (1) separation of lipids by one-dimensional thin-layer chromatography on silica gel plates; (2) elution of neutral lipids from scraped silica gel with chloroform-methanol (4:1); and (3) colorimetric determination of...
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The surface area (A) of a lipid was directly proportional to the amount of lipid in a surface film (A = k micro moles) measured at constant surface pressure, temperature, and subphase composition. A surface area of 2300 cm(2)/ micro mole was obtained for cholesterol isolated from human adrenal and aorta and for cholesterol from hydrolysates of cholesteryl esters isolated from the same tissues. ...
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An investigation of the different steps in the method of Weil-Malherbe & Bone (1949), as modified by Taylor (1953), has led to an altered procedure for citric acid determination, with increased sensitivity and accuracy. The main modifications involved are the use of (i) a higher concentration of sulphuric acid during the formation of pentabromoacetone, and (ii) a thiourea-borax-sodium sulphide ...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1963
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)40376-1